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KMID : 0357919860200040413
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Korean Journal of Pathology
1986 Volume.20 No. 4 p.413 ~ p.426
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Histochemical Muscle Fiber Types of Autopsied Human Gastrocnemius, Soleus, Peroneus longus and Tibialis anterior Muscled
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Abstract
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This study was designed for the evaluation of the mean proportions and range of
individual difference of muscle fiber types in human Gastrocnemius, Soleus, Peroneus
longes and Tibialis anterior muscles, respectively.
Flash-frozen muscle sections obtained from 15 cadavers were stained for H&E,
trichrome, PAS, regular & reversed myosin ATPase, succinic dehydrogenase(SDH), acid
phosphatase (Ac-Pase) and alkaline phosphatase (Alk-Pase).
The results obtained were as follows.
1) Differentiation of muscle fiber types was not evident in H&I and trichrome stained
specimen. Three types of muscle fibers were, however, evident in PAS-stained sections
according to the degree of positivity in observed skeletal muscles.
2) Two fiber types (Type I and Ⅱ) were only differentiated and the predominant
muscle fiber type was type I by regular myosin ATPase reaction in Gastrocnemius,
Soleus, Peroneus longes and Tibialis anterior muscles.
3) Three muscle fiber types (Type Ⅰ, Ⅱa and Ⅱb) were, however, differentiated and
the predominant muscly fiber type was type Ⅱa, contrary to patterns of regular myosin
ATPase in skeletal muscles except for Soleus muscle by reversed myosin ATPase
stains. Only two muscle fiber types (Type I and Ⅱa) and predominant type I fibers
were shown in Soleus muscle.
4) SDH stains showed three types of muscle fiber except Soleus muscle. The mean
proportions (%) of type I fibers were 48.0% in Gastrocnemius, 62.3% in Soleus, 40.8% in
Peroneus longus, and 39.8% in Tibialis anterior muscles, respectively.
5) The mean proportions (%) of muscle fiber types in human skeletal muscles were
markedly different, according to individuals and various enzyme histochemical stains.
6) The size of muscle fibers was relatevely small in type I fibers compared with type
Ⅱb.
7) Evaluation for the combination patterns of various enzyme histochemical activities
showed that the common muscle fiber types (Type Ⅰ, Ⅱa and Ⅱb) were markedly
reduced in the mean proportions and unusual rare muscle fibers predominant.
This predominance of unusual muscle fiber types and marked discrepancy in muscle
fiber types between regular and reversed myosin ATPase seemed to be ascribed to
decreased muscle enzyme activities following postmortem period.
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